1-9 of 9 Q&A
Question
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Abcam community
Verified customer
Asked on Mar 21 2012
Answer
Thank you for your reply.
The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please send me your current email address and I will generate the quote.
I look forward to your reply.
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Abcam Scientific Support
Answered on Mar 21 2012
Question
Is it possible to get Mouse IgG1 to use in Flow cytometry without being FITC labelled? I appreciate your help. Kind Regards,
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Abcam community
Verified customer
Asked on Jul 27 2012
Answer
I have found one antibody which may be of use to you.
Please have a look at the datasheet of the Mouse antibody ab59498. This antibody has not been conjugated.
I hope this information has been of help. If you need any further information or help in setting up your experiment please do not hesitate to ask.
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Abcam Scientific Support
Answered on Jul 27 2012
Question
Hi And if you could alsogive me the concentration of the Isotypr control Mouse IgG1 antibody # ab106163 that would be great? Kind Regards,
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Abcam community
Verified customer
Asked on Jul 27 2012
Answer
Thank you for your reply.
The MOUSE IgG1 antibody [MOPC-21] (FITC) (ab106163) is provided at a concentration of 1 mg/mL. This information can be found on the datasheet of ab106163 under the "Concentration" heading.
I hope this information has been of help. If you have any further questions, please do not hesitate to ask.
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Abcam Scientific Support
Answered on Jul 27 2012
Question
Yes, it is correct. ************does the purchasing in this group usually. I am a visiting professor here. Feel free to contact him to double-check, he is the one who gave me the customer ID.
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Abcam community
Verified customer
Asked on Mar 22 2012
Answer
Thank you for your reply.
I have sent the quote for the products requested to the email address we have on file for **********.
When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.
If there is anything else I can help you with, please let me know.
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Abcam Scientific Support
Answered on Mar 22 2012
Question
Concerning the quote, the customer number is**********
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Abcam community
Verified customer
Asked on Mar 22 2012
Answer
Thank you for your reply.
Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.
Thank you for your patience, and I look forward to your reply.
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Abcam Scientific Support
Answered on Mar 22 2012
Question
May I know if the following antibodies can be used for ICC and/or western blot? 1) CD31 (ab27333) 2) CD71 (ab47095) 3) CD44 (ab30437) 4) CD90 (ab11155) Also, can the IgG1(ab106163) be used as the isotype control for FACS with the 4 antibodies listed above? Many thanks in advance!
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Abcam community
Verified customer
Asked on Jan 16 2012
Answer
Thank you very much for your interest in abcam antibodies. To our knowledge,these primary antibodies (ab27333, ab47095, ab30437 and ab11155)have not been tested in Western Blot and ICC/IF. They have been tested only in Flow cytometry as shown on the datasheets. Therefore, I can offer a discount off a future purchase if you buyone or allof themnow, test it/them in Western Blot and/or ICC/IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discountwould be to the value of 1 free primary antibody for each tested antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and teston of those antibodiesin Western Blot and/or ICC/IF. I will then send a discount code. This code must be issued before purchasingthe antibodyso please wait for my reply before ordering. 2. Purchasethe primary antibody to be testedeither by phone, fax, or online (www.abcam.com). 3. Test it in Western Blot and/or ICC/IF. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe tested antibodyturns out to be unsuitable for Western Blotor ICC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. I would like to confirm that ab106163 (Mouse IgG1 conjugated to FITC)can be used asisotype control with ab27333, ab47095, ab30437 and ab11155.
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Abcam Scientific Support
Answered on Jan 16 2012
Question
Sorry for forgetting to ask another question. I noted that the application of some antibodies is only for flow cytometry.CouldI use these antibodies to do western blot orICC? Thank you!
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Abcam community
Verified customer
Asked on Jan 16 2012
Answer
If the antibody datasheets only list "flow cytometry" in the Applications section, then that is the only application that has been tested. It is possible that the antibodies could also work for western blotting or ICC, but these applications have not been tested, and they are not covered by our guarantee.
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Abcam Scientific Support
Answered on Jan 16 2012
Question
Thanks so much for your kind help. As I known, for flow cytometry, people always use normal cell(without antibody disposal) as control to set up the machine before running. But there are also some people use isotype antibody to replace normal cell as control. Which method is recommended for my control group to set up program? Many thanks!
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Abcam community
Verified customer
Asked on Jan 14 2012
Answer
A cells-only control with no antibody will show the contribution of autofluorescence of the cells themselves. Most people use this to set up the flow cytometer. An isotype control (cells incubated with the same isotype of antibody as the target-specific antibody) will include autofluorescence signal but also the non-epitope-specific interaction of the antibody with the samples. Examples of these interactions include the binding of the Fc region of the antibody to Fc receptors on the cells, and any non-epitope-specific antibody-protein interactions (for instance, hydrophobic interactions) . You could use this isotype control to adjust your flow cytomer settings but most people include it as an experimental control, after setting up the flow cytometer with cells-only. You will be able to see if there is any signal from the isotype interactions by comparing the isotype control to the cells-only control. The antibodies conjugated to FITC that you asked about are all isotype IgG1 (ab30437, ab27333, ab11155). An appropriate isotype control is ab106163: Click here (or use the following: https://www.abcam.com/FITC-Mouse-IgG1-kappa-MOPC-21-Isotype-Control-ab106163.html). I hope this helps. If you have any questions, please contact me.
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Abcam Scientific Support
Answered on Jan 14 2012
Question
What is the concentration of the current lot, GR8687-3?
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Abcam community
Verified customer
Asked on Nov 02 2011
Answer
Thank you for contacting us. The current lot of the anti-CD162 FITC conjugate, ab88033, lot *****, is 0.01 mg/ml.The isotype control we were looking at, ab106163, is 1.0 mg/ml, so you will want to dilute that 100-fold to match the concentration of ab88033. For the best control, especially if you are staining live cells that are sensitive to azide, you will want to make this dilution with the PBS buffer that ab88033 is presented in:Preservative: 15mM Sodium Azide Constituents: 0.2% BSA (high-grade, protease free), PBS.For each flow cytometry test, add 20 ul antibody per 100 ul suspension of 10e6 cells. I hope this helps. Please let me know if you have any questions.
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Abcam Scientific Support
Answered on Nov 02 2011
